We utilized a compatible 5.5 kilobase pair (kb) plasmid, pARO190 (ATCC), which contains an origin of transfer (oriT) required for conjugation from a donor to a recipient.
First, the backbone of pmT3SS includes an origin of transfer region (oriT) to facilitate the transfer of this plasmid from one strain background to another via conjugation.
E. coli conjugation donor strains are able to transfer plasmids containing mobilization genes (mob) and an origin of transfer (oriT) into G. sulfurreducens (5).
The phylogenetic tree (Fig. 1) appears to represent an origin for the calpain gene within the bacteria, followed by vertical gene transfer into eukaryotes.
It may also be in the sense of a point of origin for the transfer of information: the central dogma of molecular biology, which represents a 1950s reformulation of Weismannism in terms of information theory, asserts that information travels unidirectionally from nucleic acids to protein, and never vice versa.
The ancestral affinity of mtMutS to non-eukaryotic MutS7 provides compelling support for an origin by horizontal gene transfer.
After this characterization of the communities, half of the communities were translocated between the two sites within a region while the other half was back-transferred to their site of origin to control for transfer effects without site change.
Moreover, the large range of GC content within a bacterial order makes the use of this measure difficult for discriminating a potential origin of transfer because the phylogenetic tree usually shows distant relationships between our candidate partners.
By central architectural model we mean the physical, architectural model as the point of origin for the further transfer into a) structural, analytical software or b) into the thermal analysis software.
We describe an Australian origin for B. pseudomallei, characterized by a single introduction event into Southeast Asia during a recent glacial period, and variable levels of lateral gene transfer within populations.
Our computational studies support an archaeal origin for NucS, as previously suggested12, built upon two distinct domains that suffered a complex evolutionary history of transfers and/or losses, including at least two HGT events to Actinobacteria and Deinococcus-Thermus.
