The preparative SFE extracts were separated and purified by high-speed counter-current chromatography (HSCCC) with a selected two-phase solvent system composed of ethyl acetate n-butyl acetate n-butylt alcohol watervolume ratio of 1:4:5, and the coptimizedfractions were analyzed by high-performance liquid chromatography (HPLC).
Open image in new window Fig. 7 Different relative permeability curves used during the validation of the history matching optimization algorithm Open image in new window Fig. 8 Comparison between target (synthetic) and optimized volume of the produced fluid by using target and optimized sets of relative permeability (presented in Fig. 7).
The optimized volume of the Plus reagent (0.25, 0.5, 0.75 or 1 μl) was added to the diluted DNA.
The clear lysate was loaded into the Ni-NTA column, and washed with optimized volume of wash buffer containing 50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole.
The fabricated MRB with a diameter of 16 mm and a height of 13 mm has achieved a maximum voltage of 34.6 ± 0.4 V, an activation time of 8.6 ± 0.6 ms and a maximum capacity of 37.4 ± 0.4 W s at an optimized electrolyte volume of 180 mm3.
Micromechanical study of a representative volume element (REV) was carried out using the 3D-finite element method to optimized volume fraction of the reinforcement.
In consideration of empirical rules, including the multi-component system, an optimal concentration of small atoms, negative heat of mixing and an appropriate atom size mismatch among the main components, the cored wires were designed to contain eight elements, which have an optimized atomic volume strain criterion λn, in range of 0.14 0.21, to render the coatings a high glass forming ability.
In burn patients, optimized volume and fluid loading are of particular clinical interest since greater volume shifts are seen in these patients over a short time and empirical volume therapy frequently underestimates the real fluid requirement.
DHQase catalysis was optimized in volume of 100 μL and with various DHQ concentrations (0.08 to 1.0 mmol/L).
The influence of pH, N2B concentration and phase ratio (O/W) (organic phase volume to aqueous phase volume) were investigated for an optimized condition of xylose and glucose extraction from the aqueous solution.
