The optimized scenario exhibited an early increase in chlorophyll concentration, as compared to the other cultures, indicating a higher degree of culture health and photosynthetic potential during the exponential growth phase.
The increase in total biodiesel potential is partially attributed to increased concentrations of TAG in the optimized scenario; however, a portion also appears to have been derived from membrane-bound lipids or glycolipids.
> -wrap-foot> Experimental conditions: (1) the optimized scenario of a continuous sparge of atmospheric air supplemented periodically with 5%% CO2 (v/ v) to maintain pH between 8.4 and 8.7 and an initial addition of 5 mM NaHCO3 at inoculation plus an additional 50 mM of ACS grade NaHCO3 just prior to nitrogen depletion to stimulate TAG accumulation.
The solution reflects an optimized operation scenario for the relevant industrial organization.
A systematic approach was conducted with all the strategies to assess their potential effectiveness, according to the following steps: theoretical design, setpoint optimization and, finally, a detailed comparison of the control results against a reference operation and an optimized reference scenario.
Among all separate factors, drilling new production wells is the best one; however, even in this case the difference with the optimized scenario is high, which means that optimization plan should not be concentrated only on one of the factors influencing the oil production.
Additionally, to assess how cultures grown under the optimized scenario would perform when using a lower grade of bicarbonate, a third set of cultures was supplemented with 50 mM NaHCO3∙Na2CO3 (sesquicarbonate), instead of 50 mM ACS grade NaHCO3, to induce lipid accumulation.
This increased chlorophyll content along with the higher carbon fixation rates indicates a higher photosynthetic performance of the alga when cultured under the optimized scenario; however, additional photosynthetic parameters will need to be monitored in the future to verify this interpretation.
The organic and inorganic phosphorus loads decreased by 3.6%and3.7%7%, respectively, in the optimized scenario compared to those in the historical trend scenario.
In contrast, under the optimized scenario (genetic algorithm (GA) prediction), up to 3370 ha of dryland area was converted into forest area.
