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Therefore, it is possible that an optimized expression of BR-related genes could increase grain yield in rice.
Our findings define the functions of D2/SMG11 in grain size and grain yield, suggesting that an optimized expression of D2/SMG11 is a promising approach to improve grain yield in rice.
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Here we describe the design and construction of an optimized expression and purification system for the full-length, human Cullin3 RINGBox 1 (Rbx1) protein complex from Escherichia coli.
An optimized expression system capable of producing from 25to3535 mg of biologically active hER-E/F in 1 liter of cell culture was designed, and a simple, rapid purification protocol for hER-E/F produced in this system was developed.
To better understand this, we characterized mutations that optimized expression of a plasmid-borne metabolic pathway during laboratory evolution of a bacterium.
Successful quantitative trait locus (QTL) introgression depends on the optimized expression of newly introgressed QTLs in the recipient genome background, with the aim of maximizing productivity.
Optimized expression of Hsf1p is important for yeast cells because either the deletion or overexpression of this gene leads to growth arrest [ 15].
For codon-optimized expression of Drosophila FDL, a P. pastoris codon-optimized sequence was custom synthesized by GenScript, USA.
Optimized expression enabled recovery of purified fucosyltransferases in a yield of up to 45 mg/L culture for α1,2-fucosyltransferases and of up to 200 mg protein/L culture for α1,3/4-fucosyltransferases.
The optimized expression and purification of Ac-AChBP in Bac-to-Bac baculovirus system had also applied for expression of another similar protein Ls-AChBP (GeneBank number AF364899.1) and achieved very good results similar to those of Ac-AChBP (data not shown).
In this report, we describe the development of an optimized direct expression system, designed to maximize the extracellular accumulation of recombinant glycine-extended salmon calcitonin peptide (sCTgly).
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