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Within the headquarters is an optimized blend of collaboration space and natural features.
Briefly, 1 µg of total RNA was added to a 20 µl reaction mix containing reaction buffer, magnesium chloride, dNTPs, an optimized blend of random primers and oligo dT), an RNase inhibitor and a MMLV RNase H+ reverse transcriptase.
cDNA was synthesized from the total RNA extracted from different individuals at different times using the Qiagen Quantitect RT kit which uses an optimized blend of oligo-dT and random primers to promote high cDNA yields, even from 5′ regions.
Briefly, 2 μg of RNA were added to a 40 μl reaction mix containing reaction buffer, magnesium chloride, dNTPs, an optimized blend of random primers and oligo dT), an RNase inhibitor, and a MMLV RNase H+ reverse transcriptase.
Briefly, we use an optimized blend of Al nanoparticles (fuel) in a fluoropolymer (oxidizer) matrix that is spray-coated onto the surfaces, and which burns away extremely rapidly (< 1 sec/cm), generating very high temperatures [2200 3200KK [ 18]] that destroys the biofilm, but leaves the underlying surface intact.
An optimized blend of surfactants and electrolytes can significantly retard defect evolution due to molecular bridging.
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The Rest Easy website says that its "optimized blend of natural ingredients has been universally recognized for thousands of years as a means for controlling insects". In a phone interview, company President Howard Brenner said, "We are all-natural and highly effective". He also said that people who have a serious and obvious infestation should call an exterminator.
For example, doubling nonanal (13%) in the optimized blend of 0.1 µg/µl would result in 0.113 µg/µl concentration of the nonanal doubled blend a 13% increase in total concentration.
To test for the effect of ratio, we modified the optimized blend by doubling the amount of each component singly, while holding the amount of the remaining compounds constant.
To decouple the effect of changes in ratio and concentration, we first manipulated the ratio of the "optimized blend" (Table 1) according to the volatile ratio released from damaged grape shoots and prepared a "damaged shoot blend" (JB damaged blend in Table 1).
We confirmed this by using a previously identified 7-component optimized blend [23], and testing various ratio-modified (systematically doubling single compound) synthetic blends in the flight tunnel (Fig. 1) and also by mimicking increased and decreased ratios of the constituents in the optimized blend as emitted from grapevines damaged by one day of Japanese beetle feeding (Fig. 3 and 4).
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