The phrase "an optimised one" is correct and usable in written English.
It can be used when referring to something that has been improved or made more efficient, typically in a technical or performance-related context.
Example: "We have developed an optimised one that significantly reduces processing time."
Alternatives: "an enhanced version" or "a refined model".
For all optimised one-step or two-step PCR assays, the interassay CVs were lower than 10%, demonstrating good reproducibility for all tested kits [ 23].
In this work, we systematically compared 3 different digestion protocols and optimised one, reaching 100% dissolution within a very limited time (1 h) together with the requirement of only a few milligrams of sample, and safe experimental conditions.
In simulation, a hemispherical cup of diameter 100 mm was considered and simulation studies were carried out for all the possible locations of the drawbead, and location which gave minimum value of major principal strain is taken as the optimised one.
The optimisation procedure included establishing the concentration of magnesium chloride (from 2 to 3 mM), reaction buffer (Fermentas, Vilnius, Lithuania) used for amplification (supplemented with 50 mM KCl or with 20 mM NH4SO4), the ratio between used primers (from 1 1 1 until the optimised one) and last but not least, the protocol for amplification.
We found that the optimised one-step PCR assays had a higher detection sensitivity than the optimised two-step assays regardless of the machine used, while no difference was detected in reaction efficiency, R values, and intra- and interreproducibility between the two methods.
Model performance varied considerably for the fen soil profile under grassland use, where the mean normalised root mean square error (RMSEN) was 16.0% for the original parameters and 52.9% for the optimised ones.
The standard threshold used for the 3.5 ms change was 7 μV, whilst the optimised ones ranged from 4 9 μV.
The two other optimised one-step assays (i.e., Verso-1 step and qScript-1 step) revealed poor sensitivity with a detection of 10 and 10 copies/ µL, respectively.
Of the five one-step kits tested using the Mx3005P cycler, three optimised one-step kits (i.e., Quantitect-1 step, Rotor Gene-1 step, and SSIII Platinium-1 step) revealed good sensitivity with the detection of at least 10 copies/ µL.
The aim of this work was to show how quality assessment should be carried out in order to transfer an optimised method from one technique to another.
