Photobleaching was performed using the 488 nm line from an Argon laser (measured through the 10× objective) operating at ∼3,8 mW, collecting emission between 500 and 600 nm.
Specimens were imaged by confocal fluorescence microscopy using a Zeiss LSM 510 microscope fitted with a Zeiss ×63, numeric aperture 1.4 objective operated in single photon mode, with standard filter sets verified for lack of detectable cross-channel bleed-through and standard (1 Airy disc) pinhole.
Images were acquired with an Axiovert 200 M microscope/EC Plan-Neofluar ×40/1.3 oil immersion or C-Apo ×63/1.20 water immersion objective equipped with a charge-coupled-device camera (all from Carl Zeiss MicroImaging GmbH) or an LSM 710/Axiobserver Z1 confocal microscope ×63/1.4 oil immersion objective operated by ZEN 2009 software.
This objective operates throughout medical training and subsequent clinical practice and is a continuous process.
Instead of describing their solutions, companies first need to understand customers' specific challenges, objectives, operating practices, and competitive environment, then create offerings to deliver value within a customer's specific business context and culture.
Here, process objectives, operating principles, reactor designs, parameters for process monitoring and control, and their effect on biodried output quality are critically examined.
Such a decision-making is a problem involving multi-criteria decisions, and requires a logical and objective operating procedure.
Moreover, the CoP must have clear objectives, operate in an enabling environment that will generate trust, the technology platform must be user-friendly and there must be transparency and feedback to participants about their ideas.
Table 1 Examples of educational mobile games Game Pedagogical objective Operating system Edugame (Hssina et al., 2014) Teaches construction of words, recognition of letters or mathematical operations Android Geometry game (Ketamo, 2002) Teaches geometry iOS.
Task scheduling is a main objective in operating system design.
