Sentence examples for an light from inspiring English sources
Figure 1 Network components in an Internet building block representing a hop. Figure 2 Network components in an light path building block representing a hop.
In a 25 µl PCR reaction, 2 µl of cDNA (corresponding to 20 ng of total RNA input) was amplified in an Light Cycler 480 (Roche) using 2-fold QuantiTect Multiplex PCR Master Mix (Qiagen), 50 nM primers and 100 nM probe for the 18S rRNA reference gene (fwd: 5'-CGGCTACCACATCCAAGG-3', rev: 5'-CGGGTCGGGAGTGGGT, probe: 5'-TTGCGCGCCTGCTGCCT), and 300 nM primers and 100 nM probe for the gene of interest.
Microconidia were visible under an light microscope (Fig. 2c).
Japanese standard number 3 size (235 × 120 mm) envelopes of an light yellow green color were used.
The solution was stirred at room temperature for 4 h, then the solvent removed under reduced pressure and the crude product purified via silica column chromatography (5% MeOH in CH2Cl2) to yield the Boc-protected intermediate, tert-butyl 2- 4- 2,3-bis((Z)-octadec-9-enyloxy)propylamino)-4-oxobutanamido)ethyl carbamate (998 mg, 79%) as an light orange oil.
