Consistent with the protein-coding genes, these substitutions consist mainly of A to G (36%), C to U (43%) and U to C (14%) substitutions, with one case of C to G. Given that dinoflagellate mitochondrial genes occur in multiple copies, recovery of further, independently isolated copies of these genes will be required to substantiate these inferences of rRNA editing.
It is certainly the case that a professor or student — like anyone else — may on an isolated occasion copy a small amount of material and successfully assert fair use.
Our results indicate that CNVs detected by arrays may be the coincidental co-localization of smaller CNVs, whose presence is more likely to perturb an aCGH hybridization profile than the effect of an isolated, small, copy number alteration.
However, the transposition of an isolated ESAG7 copy into an active expression site (without ESAG6 and 5) has been reported under in vitro drug and serum selection pressure [38].
Complex CNVs may simply be the coincidental co-localization of smaller, simpler CNVs, whose presence is more likely to perturb the aCGH hybridization profile than an isolated, small copy number alteration.
"In order to see if this finding was real, we wanted to isolate copies of the genes ourselves," Taylor says.
Eight 'high copy' isolates (with copies varying from 7 to 17) along with two single copy isolates formed Cluster 3.
To fill this gap, our objectives were to 1) study the interspecific distribution of BuT5 using both bioinformatic and experimental methods; 2) isolate a copy of the autonomous element that mobilizes BuT5; and 3) classify BuT5 and its master TE.
Also, the average nucleotide heterozygosity at the hsp82 locus was 0.0016, compared to a much higher average nucleotide difference of 0.024 among mmr-b copies within an isolate, suggesting multiple copies homogenized by concerted evolution rather than allelic variation.
