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To control for the plausibility of the results, the three-path mediation models were re-calculated with an inverted sequence of mediators.
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This construct was delivered in a 1 1 ratio with AAV constructs bearing an inverted sequence – indicated by a flipped (leftward) arrow – of the gene of interest under control of CMV promoter (CMV – white arrow).
Congruent stimuli could have consisted of an inverted sequence (high-ranking aggressor scream then low-ranking victim scream).
As the satellites found in each species are related to each other (e.g., dmel. Satellite 360 contains a partial inverted sequence of the other two satellites), I further used MEME to identify monomers of the same family.
The inverted sequence of SE24C2C2SE24 results in an extra complicating factor for nucleation, namely the meeting of the two ends of one molecule.
The inverted sequence of stimuli resulted in a static percept.
Accordingly, an evolved candidate (Cl2B) with 4 mutations in the inverted sequence of the λCl repressor binding (Additional file 1: Figure S6B) was obtained.
An adenovirus containing the GFP gene and the inverted sequence of human UCP2 cDNAwas used as control (Adcontrol).
For the DAT-KO control group, the AAV construct bore the tdTOMATO gene sequence (CMV-DIO-tdTOMATO-AAV, magenta arrow), while for the DAT-KO treated group, the AAV bore the inverted sequence of the mouse Dopamine Transporter (mDAT) gene, depicted with a teal arrow (CMV-DIO-mDAT-AAV).
mt1 and mt2 of Hydra share almost identical but inverted sequences of 191 196 bp at each 5' and 3' end (inverted terminal repeats (ITR), Figs. 1A, B, see below).
Peak lists were created using DTASuperCharge [49] with default parameters and searched using Mascot (v2.2) against the Honey Bee, A. mellifera Amel_4.0 translation (forward plus inverted sequences) of the genome with additional entries for human keratins, porcine trypsin and LysC.
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