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This represents an initial tolerance checkpoint, with the vast majority of the newly generated B cell repertoire not surviving to exit the BM, 32 with cell death occurring through apoptosis.
Features from the LC-MS analyses were matched to AMT tags to identify peptides, using an initial tolerance of ±3 p.p.m. for mass and 2.5% for the LC normalized elution time (NET).
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One missed cleavage per peptide and an initial mass tolerance of 10 ppm were used in all searches.
One missed cleavage per peptide was allowed, and an initial mass tolerance of 50 ppm was used in all searches.
The proteins were unambiguously identified by searching against a comprehensive non-redundant protein database using the Pro-Found and MASCOT programs [19]; one missed cleavage per peptide was allowed, and an initial mass tolerance of 50 ppm was used in all searches.
The search was performed with an initial mass tolerance of 6 ppm mass accuracy for the precursor ion and 0.5 Da.
The search was performed with an initial mass tolerance of 7 p.p.m. mass accuracy for the precursor ion and 0.5 Da for the MS/MS spectra obtained with CID fragmentation and 20 p.p.m. for the MS/MS spectra in the HCD fragmentation mode.
One author, Rudolph Sabor, sees a link between the Oceanids' treatment of Prometheus and the Rhinemaidens' initial tolerance of Alberich.
The initial tolerance curve predicted whether head injury would occur as a function of the head impact duration and the average linear acceleration measured at the occiput.
However, B cells expressing these receptors take time to disappear, suggesting that anergy is the initial tolerance mechanism (T. Kawahara et al., unpublished observations).
We find that 98.5%, 96.9%, and 92.0% of branches were matched to within the original tolerance for (epsilon_{0}=0.1500, 0.0750,mbox{and } 0.0375) respectively - even though we allow this tolerance to fall off if a matching tree is not found after (N_{mathrm{t}}=50) trials it is clear that the majority of cases are actually matched at the required initial tolerance.
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CEO of Professional Science Editing for Scientists @ prosciediting.com