Sentence examples for an initial denaturation for from inspiring English sources

After an initial denaturation for 3 min at 95°C, 43 cycles of denaturing at 95°C for 30 s, annealing for 30 s and extension at 72°C for 1 min were performed, followed by a final extension at 72°C for 10 min.

The PCR program consisted of an initial denaturation for 1 min at 95°C; followed by 35 cycles of 10 s at 95°C, 10 s at 55°C, and 1 s at 72°C; and with a final extension for 30 s at 72°C.

The cycling program consisted of an initial denaturation for 1 min at 95°C, followed by 30 to 40 cycles of denaturation at 95°C for 15 s, annealing for 15 s, and extension at 68°C for 45 s, with a final extension of 1 min at 68°C.

The PCR was carried out using Bio-Rad thermal cycler with an initial denaturation for 5 min at 94 °C, followed by 35 cycles for denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, extension at 72 °C for 1 min, and a final extension at 72 °C for 10 min.

The DNA amplification protocol included an initial denaturation for 3 min at 95 °C, followed by 35 cycles of denaturation for 30 s at 95 °C, annealing for 90 s at 55 °C, and an extension for 90 s at 72 °C, with a final extension for 10 min at 72 °C.

The PCR protocol entailed an initial denaturation for 10 min at 94 °C; 25 cycles of denaturation for 45 s at 94 °C, annealing for 30 s at 52 °C, and elongation for 90 s at 72 °C, followed by a final 5-min elongation at 72 °C.

After an initial denaturation step for 1 min at 94°C, conditions for cycling were 35 cycles of 30 sec at 94°C, 30 sec at 56°C, 1 min at 72°C.

After an initial denaturation step for 10 min at 95°C, each cycle consisted of denaturation for 15 s at 95°C and annealing and primer extension for 60 s at 60°C.

PCR was performed with an initial denaturation step for 10 minutes at 95°C followed by 40 cycles at 95°C for 15 seconds, 55°C for 30 seconds and 72°C for 30 seconds.

The cycling conditions were an initial denaturation step for 15 minutes at 95°C followed by 45 cycles of repeating denaturing at 15 seconds at 95°C, annealing for 30 seconds at 55°C, elongation for 30 seconds at 72°C.

Samples were amplified (Biorad Tetrade, Hercules, CA,USA), following an initial denaturation step for 10 min at 94°C, by 32 cycles of 30 s denaturation (95°C), annealing for 30 s (57°C) and elongation for 30 s (72°C).