Sentence examples for an initial assembly from inspiring English sources

Assembly 2 is an initial assembly at a higher stringency of 1×10-60 with 17,832 contigs.

We used the command line CLC bio Assembly Cell (Aarhus, Denmark) to generate an initial assembly due to the small memory footprint of this assembler.

A de novo genome assembly of the shotgun, 454 and Illumina (pseudocontigs) sequence data was performed using the Roche/454 Life Sciences Newbler (Gs) assembler v 2.3 [ 69], producing an initial assembly of 102 contigs (>500 bp) distributed over 32 scaffolds for the genome of JCM1046.

During an initial assembly PCR, these oligos function as both primer and template, while the DNA polymerase successively builds longer and longer fragments with each round of PCR.

2. After an initial assembly of the reads, often fosmid libraries are employed to accomplish the finishing of a genome.

We develop SEQuel, a tool that refines an initial assembly of short-read data by using approximate positions of reads in contigs.

Finally, GapResolution and dupFinisher programs [ 62, 63] were used to close gaps and correct mis-assemblies to generate an initial draft assembly, which contained 14 scaffolds composed of 247 contigs, and 12 scaffolds composed of 115 contigs for RM13514 and RM13516, respectively.

In practice, contigs are assembled in several steps, including initial assembly at a high strigency, DQing questionable clones at an increasing stringency, and end-to-end merging and singleton-to-contig end merging at decreasingly lower stringencies.

Both Nup96 and Nup160 (yeast homologs are Nup145C and Nup120, respectively) are components of the conserved Nup107−160 complex that has a role in the initial assembly of the NPC and functions as a stable anchoring point for other Nups referred to as central scaffold Nups (Walther et al. 2003; Rasala et al. 2006; Grossman et al. 2012).

Taken together with previous findings this suggests, that MTA-RBBP is a stable complex, with a central role in the initial assembly of the human NuRD complex.

The initial assembly used a Sulston score of 1× 10-45 and a tolerance 0.4 bp.