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The alignments generated by Prospero were used as an initial alignment to make profile Hidden Markov models using HMMER [ 59], and the resulting alignments were scanned against the Swiss-Prot database.
We shifted the Illumina base quality scores (Phred+64) to the Sanger scale (Phred+33) [ 6] and performed an initial alignment to the National Center for Biotechnology Information human reference genome HG19 using the program Burrows-Wheeler Aligner (BWA) [ 7].
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The pharmacophore alignment function of MOE was used to produce a rough initial alignment to ATP of the diverse input set based on common chemical features.
Duplicate reads were removed after the initial alignment, to eliminate reads that mapped to the same position of the transcriptome.
To confirm homology between the procaryotic piwi mid domain and the Ago family of sequences and to establish an initial alignment, a sequence corresponding to the mid domain of the A. aeolicus Argonaute structure ([PDB: 1yvu], range 349 484) was used as a PSI-BLAST query (E-value cutoff 0.005, maximum 20 rounds, NR database).
The method first looks for small fragments within matched SSEs to make an initial alignment, and then extends the alignment to the rest of the residues by considering a penalty for each gap.
This method takes an initial alignment and constantly makes changes to it.
(A ) Initial alignment of the injectisomes to the common origin.
This noise amplitude is gradually decreased to zero, starting from some initial value T and an initial alignment of reciprocal best sequence matches.
The rigid registration process involved an initial alignment of the surface centroids followed by an iterative closest point (ICP) step (Besl & McKay 1992) to refine results.
Instead of just using the BLOSUM-62 matrix to compute alignment scores, PSI-BLAST computes substitution scores column-by-column, based on an initial alignment and subsequent refinements.
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