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Subconfluent cells were treated with the compound at indicated concentrations for an indicated time.
For H2O2 treatment, HCT116 cells were treated with 100 μM H2O2 for an indicated time.
For detection of apoptosis, HeLa cells were seeded onto 12-well plates and treated with etoposide (20 μM) or taxol (0.1 μM) for an indicated time.
For insulin stimulation, cells were incubated with serum-free DMEM for 16 hours followed by a treatment with 100 nM insulin for an indicated time.
A suitable promoter for efficient production of recombinant gene products is considered based on its strength and controllability (i.e. inducibility) at an indicated time or condition (Timmis et al. 1994).
After an indicated time (24, 48, or 72 h), the in vivo brain elimination experiments were performed using the intracerebral microinjection technique [ 25].
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Cells were incubated for an additional indicated time.
Wild-type, MUL1 knockout, and PINK1 knockout (as a control) HeLa cells were transfected with YFP-Parkin and treated with DMSO or antimycin A for indicated time.
At indicated time points, an equivalent of 6·10 cells was transferred into a 96 well plate and harvested by centrifugation.
(A – A′ ) Quantification of Atg5 punctae formation in S2 cells incubated with rapamycin (A ) and incubated with KRB with or without wortmannin (A′ ) for indicated time points.
There is a lighted candle (indicating that the time of day is dusk), a metronome (indicating time itself), and two earlier Matisses: a small sculpture of a sensual odalisque and a large image from a painting of a stern-seeming woman seated on a high stool, floating free on the gray wall.
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CEO of Professional Science Editing for Scientists @ prosciediting.com