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Experimental steps of transduction of breast cancer cells with adenoviral vectors can be summarized as follows: Breast cancer cells were infected with an increasing multiplicity of infection (MOI) of AdEGFP (vector expressing enhanced green fluorescent protein (EGFP) reporter gene) vector at 37°C in RPMI 1640 without FBS.
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That is, an increased multiplicity of available solutions has a clear positive effect on efficacy.
The enhanced ability of T/F viruses to infect MDMs via cell-to-cell spread from infected T cells might be explained by an increased multiplicity of infection (MOI) imparted by this mode of spread.
We repeated the experiment using live EBV and as observed with poly(I C), a single monocyte treatment with increasing multiplicity of infection (m.o.i).o.i
In support of this explanation, we observed similar levels of TNF in the supernatant of Cd36 +/+ and Cd36 -/- thioglycolate-elicited peritoneal macrophages infected with mycobacteria in vitro, which increased in a dose-dependent manner with increasing multiplicity of infection with both M. marinum and BCG.
To overcome these deficiencies, a new method called customized redundant multiwavelet (CRM) is constructed via increasing multiplicity (IM).
As more is known about individual scales single neuron, circuitry, behavior more challenges arise as to how to connect the pieces, because of the increasing multiplicity of pathways.
In order to realize the accurate detection of the condition feature and multi-resolution analysis in the whole frequency band, adaptive multiwavelet basis function is constructed via increasing multiplicity and then vibration signal is processed by the ensemble multiwavelet transform.
Further, fluorescence levels increased along with increasing multiplicity of infection (MOI) (data not shown).
Macrophages were infected with BCG at increasing multiplicity of infection based on optical density (0.1OD600 = 5×107 bacterial/ml).
After overnight starving of FCS, monolayers were stimulated either basolaterally with TNFα (10 ng/ml) and/or apically with Bb-CM or Bb at increasing multiplicity of infection (MOI: 10, 50 or 100), i.e. number of bacteria per epithelial cell.
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