As a result, H8 cells with a stable transduction of pWPI-hId1 showed an increased colony formation ability compared to the control plasmid pWPI (colony numbers: 75.4 ± 12.2 vs 48.4 ± 7.5, P < 0.05, Fig. 4B,C).
Substantial intracolonial variation in worker physiology and behavior is thought to play an important role in increasing colony function and productivity through task specialization [8], [26], [27].
We compared the quantitative diploid data across the three quantitative traits (flocculation, invasion, adhesion) with the complex colony morphology scores and observed a weak trend of increasing colony morphology associated with increased invasion, similar to the haploids.
Colony formation assay revealed a markedly increased colony number and size by the overexpression of CENP-A (Figure 4B).
Moreover, hypoxic and DHP-d-induced de-ATSC showed a 2-fold increased colony forming unit (CFU) and increased synthetic DNA and over two-fold increased telomerase activity (Figure 1A, 1B, Fig. S2B).
These results suggest that the absence of fudoh in SCCmec type-IV is a plausible cause of the increased colony spreading, exotoxin production, and virulence of CA-MRSAs.
Moreover, overexpression of ectopic β1 significantly increased colony formation, a hallmark of transformation.
Consistent with this observation, Meis1 augmented the efficacy of HOXA1 on tumorigenic induction of primary hematopoietic cells, resulting in a more intense growth and increased colony number in soft agar assays [ 21].
Chronic cadmium exposure increased colony formation and cellular invasion.
In HEK293 cells, pEPito- [CMV-IEP]- [EGFP-IRES-BSD] exhibited a ~70 fold increased colony-forming efficiency over its pEPI-ΔMARS-control, followed by a ~45 fold increase within pEPito- [hCMV/EF1P]- [EGFP-IRES-BSD], indicating a positive effect of the MARS sequence in the pEPito vector backbone on colony-forming efficiency.
