Interestingly, the expression level of total and surface Ninj1 in MLE-12 cells, a type II pneumocyte cell line, also increased (Supplementary Fig. S5C,D).
Within the lymphocytes, T cells were relatively increased (Supplementary Figure S1), mainly due to an absolute reduction in B cells and natural killer cells (Supplementary Figure S2).
As expected, an increase in fluorescent signal was observed when the number of combined probes increased (Supplementary Figure 1D).
The dye rejection properties improved as the number of depositions increased (Supplementary Fig. 26).
We also observed that, with the increase of phenylalanine concentration from 0 to 5 mM, the biomass accumulation increased (Supplementary Fig. 1).
In YAP (5SA), but not YAP (WT), liver at days 3 7 post-HTVi, the proliferation of mainly Myc-negative hepatocytes was increased (Supplementary Fig. 3b d).
But the methylation levels of the CpG sites or genes were relatively stable as the AMD increased, (Supplementary Fig. S2d-e).
Accordingly, also the levels of Cyt-c in cytosolic extracts were increased (Supplementary Figure S2B).
Following heat shock, both HSF1WT and HSF1S121A proteins slightly increased (Supplementary Fig S5C).
Among several miRNAs, miR-532-3p wasubstantiallyly increased (Supplementary Figure S4a).
When comparing the resulting consensus network to networks obtained from the Ingenuity Pathway Analysis tool (IPA: www.ingenuity.com), we observe a steady increase in precision as the number of votes required for an edge increases (Supplementary Fig. S9), culminating at eight votes as predicted by the model in Equation 6.
