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Overexpression of miR-145 in PC3-transfected cells resulted in increased apoptosis and an increase in cells in the G2/M phase, as detected by flow cytometry.
FACS analysis was used to investigate whether the upregulation of sialoadhesin represents an increased expression per cell, or an increase in cells expressing sialoadhesin.
Figure 7 shows an increase in cells in the sub-G0/G1 phase (P1) with an increase in fragmented DNA.
For HT29 there was a clear change in cell cycle characteristics, with a dose-related decrease in cells in G1 at all time points, an increase in cells in S phase at all time points, and a small change in the number of cells in G2 compared to the control.
In line with this we do see reduced tumor cell cycle progression in Bmi1−/−BXB11 tumors and an increase in cells undergoing apoptosis albeit with some delay.
We observed cellular detachment and apoptosis concurrent with an increase in cells with a differentiated phenotype after deleting Ring1b in Ring1b−/Lox CreERT2 ES cells, but not in similarly treated Ring1b+/+ CreERT2 control ES cells.
While PIN lesions showed an increase in cells undergoing apoptosis (see above) we did not find increased levels of apoptosis, or cell proliferation, in regions of normal appearing prostatic epithelium in the Lo-MYC or Hi-MYC mice.
FACS analysis on the 199bSC1 clone showed a 31% decrease in S-phase fractions, and an increase in cells in G0-G1 of 15%, as compared to the empty vector clone (Fig. 1A).
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The PEMFC performance increases with an increase in cell temperature.
In addition, SF/HA scaffolds showed an increase in cell proliferation compared to pure SF scaffold.
During photovoltaic energy conversion, thermal energy is also generated resulting an increase in cell temperature.
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