Based on these limitations, we deep-sequenced cDNA libraries of soybean cultivar Williams 82 inoculated with P. pachyrhizi along a time-course of infection using the Illumina platform to generate a more in-depth transcript abundance profile of the genes expressed by soybean and P. pachyrhizi simultaneously.
The recent advent of RNA sequencing technology (RNA-Seq) provides an opportunity to conduct in-depth transcript profiling for heterosis studies.
The recent advent of RNA-Seq, a next generation sequencing technology, provides an opportunity to expand the identification of Stevia genes through in-depth transcript profiling.
The recent advent of RNA-Seq, a massively parallel sequencing method for transcriptome analysis, provides an opportunity to expand the identification of alfalfa genes and polymorphisms, and conduct in-depth transcript profiling.
We performed an in-depth analysis on orf21 transcript and on its product, RPHM21 (from R. philippinarum Male mitochondrial protein 21): we localized orf21 transcripts by in situ hybridization (ISH), and synthesized a specific antibody to verify the existence, by Western blotting and confocal microscopy, of RPHM21 in adults and early embryos.
An in-depth analysis of transcripts located in this cluster revealed that 27 heat shock proteins were present, comprising approximately 13% of the genes in cluster 3, and representing more than one third of all annotated heat shock proteins in V. vinifera (Additional file 1).
We created an in-depth probe/genome/transcript annotation using the AceView transcript database.
PAREsnip program was utilized to validate the different expressed miRNA/target interactions and make an in-depth survey of cleavage sites in transcripts and corresponding small RNAs.
After an in-depth reading and rereading of the transcripts, initial coding (conducted by TK) identified groups of meaning within the text.
Then a thematic analysis was conducted to produce an in-depth description and understanding of the transcripts from the focus groups' open discussion and go-along interview [ 30, 31].
