Notwithstanding these efforts, no published hypothesis of phylogenetic relationships across characiforms based on an in-depth sampling of their morphological diversity is available.
As fine roots are an important component of the below ground biomass [7,15,40] and fine root volumes were not accurately assessed using the methods described in section 2.4, an in-depth sampling of fine root biomass was carried out to coincide with the destructive harvest during the second rotation in July 2013 at both field sites.
While fragmentation is still an issue, the non-biased cloning-free transcript sampling using the Roche/454 technology combined with the conventional Sanger technology in this study enabled an in depth sampling of over 93% of the A. caninum transcriptome.
We pooled it with two other libraries, Lib1390 (hESC cell line H1 over 31 passages) and Lib1313 (hESC cell line H7 over 33 passages), to represent a more in-depth sampling.
Cohort retention is a key factor in the successful delivery of such a study and with in-depth sampling protocols, participant engagement, comfort and feedback are key factors in optimising cohort retention and comprehensive data collection.
Silene was targeted for this in-depth sampling of species-level mitochondrial divergence because of a priori knowledge of the rate acceleration in S. noctiflora.
It can be adapted for varied conditions as greater depths, more vertical levels of simultaneous in-depth sampling or large volumes.
Due to in-depth sampling, we obtained 22,740 eukaryotic ribo-tags, with more than 80% of the tags having a taxonomic resolution at least to the kingdom level.
More in-depth sampling and functional analyses of these genes in the future may reveal more molecular details concerning the adaptation of the Tibetan population to high-altitudes.
Based on these results, there is an appearance of geographically distinct populations; however, due to the limited sequence divergence between the observed groups, more in-depth sampling is needed for this to be verified.
Although the Swinomish Tribe possessed the infrastructure required to develop an in-depth environmental sampling, analysis, risk management, and education plan with a significant cultural component, they were unfamiliar with the complexities of a federal grant application.
