To investigate the chemical, structural and biological features of miscanthus biomass as a lignocellulosic feedstock, and to unveil how these characteristics vary among different genotypes, we embarked on an in-depth cell-wall analysis of 25 miscanthus genotypes from a larger replicated field trial comprising 244 genotypes.
Although microcontact printing allows for an in-depth analysis of cell responses to ligand patterns roughly the size of focal adhesions, an understanding of how cells respond to ligands presented at smaller length scales could elucidate more specific interactions of integrins with hierarchically structured proteins in the ECM.
These drug development efforts required an in-depth understanding of cell-based observations which drive the development of compound structure-activity relationships.
We characterized three ovarian adenocarcinoma cell lines, OVCAR3, CaOV3 and ES2, as well as ovarian cancer cells enriched from ascites fluid with an in-depth proteomic analysis of whole cell lysates, the cell surface proteome and proteins released into the extra-cellular compartment.
To study the surface proteome of OS cells, we used an in-depth, high-resolution approach combining cell surface biotinylation for the isolation and retrieval of plasma membrane proteins, gel fractionation and mass spectrometric analysis using nano-liquid chromatography – tandem mass spectrometry (nanoLC-MS/MS -based proteomics.
The development of technological routes to convert lignocellulosic biomass to liquid fuels requires an in-depth understanding of the cell wall architecture of substrates.
The model calculates energy usage, amino acid and nucleotide usage, membrane transport, RNA synthesis and destruction, and protein synthesis and destruction for 33 proteins to give an in-depth look at the cell cycle.
However, we do not have any evidence this is taking place as it would require an in-depth analysis of satellite cell activation in a larger cohort of calpain 3 null patients, due to the fact that activated satellite cells in vivo are only detectable within a few days.
Our finding that the blood cells are fenestrated in a functional network of Laminin A and Pericardin raised the speculation that these sites might function as bone marrow-like tissues in adult flies and thereby demanded an in-depth analysis of the cell types present therein.
We have undertaken an in-depth analysis of the cell-biological effects of PGE2 on 4 commonly employed CRC cell lines.
In a separate set of experiments, a team led by Dr. Joshua Rabinowitz at Princeton University took an in-depth look at how cells, both healthy and cancerous, could use lactate.
