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A single-chain Fv fragment (scFv) against estradiol-17β (E2) was generated to begin the construction of a library of various mutated anti-steroid antibodies with an improved affinity and/or specificity.
Remarkably, the N367I mutation completely abolishes D-glucose uptake and allows D-xylose uptake with an improved affinity.
Here, a biparatopic anti-EFGR nanobody showed an improved affinity for its target and an inhibition of tumor proliferation [57].
Following NTP binding and conversion to the catalysis-competent ternary complex, the polymerase revealed an improved affinity for the template.
Of all the created mutants, the only mutant that showed an improved affinity for NADH KMM, NADH) while at the same time it displayed a decreased affinity for NADPH KMM, NADPH) was H220Q.
Similar(55)
With compound 1b, a new ligand with a twofold improved affinity to the best so far known d-GalNAc was identified.
The derivatives with cyclized (rigidified) elements in the spacer do not have a much improved affinity [35].
In general, introduction of a large lipophilic group improved affinity, whereas functional activity often followed the opposite trend.
The Hxt36-N367A variantransportsts D-xylose with a high rate and improved affinity, enabling the efficient co-consumption of D-glucose and D-xylose.
Two modified proteins with greatly improved affinity for xyloglucan, a key polysaccharide abundant in the plant kingdom crucial for providing plant support, were generated.
Furthermore, [natGa]NOTA-pentixafor had already shown improved affinity towards CXCR4 in a previous study [21].
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