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After standard cell culture of HGFs on these substrates, attached cell number, cell spreading area, immunostained ECM including fibronectin (FN) and type I collage, and vinculin (presenting the formation of focal adhesion plaque) were quantified by morphometric analysis using an immunofluorescence microscope.
and visualized with an immunofluorescence microscope (Leica DM6000).
Some specimens were examined under an immunofluorescence microscope.
Bound antibodies were detected by using FITC-conjugated anti-human antibodies and observation under an immunofluorescence microscope.
The results were observed under an immunofluorescence microscope for double confirmation before the samples were analyzed by flow cytometry.
Inspection of cells in an immunofluorescence microscope followed by flow cytometric analysis confirmed expression of Venus in a subset of cells transfected with the VLV plasmid alone that was reduced when co-expressed along with a lacI-encoding plasmid (Figs. 1b,c).
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Images were acquired with an upright immunofluorescence microscope (BX51WI, Olympus, USA).
Samples were mounted (AF1, Citifluor Ltd., UK) and visualised using a confocal immunofluorescence microscope (Zeiss LSM 510 based on a Zeiss Axiophot M100).
Images were acquired using a confocal immunofluorescence microscope using ×100 magnification (LSM710, Zeiss).
Images were analyzed using a Zeiss immunofluorescence microscope.
The presence of ER staining was visualized under a confocal immunofluorescence microscope (Olympus, Japan).
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