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Therefore, a sequential approach with AN followed by exemestane is worth pursuing, even upon the availability of newer compounds such as fulvestrant and trilostane.
Compounds were analyzed on a C-18 column using a 6 min gradient from 1.5 to 5.0% (v/v) acetonitrile (AN), a 2 min gradient using 5%77% AN, followed by a 7 min gradient from 7% 25% AN and a 2-min gradient transitioning from 25%922% AN, then 6 min at 92% AN, then 1 min from 92%–1.5% AN and lastly 5 min at 1.5% AN.
According to Frey et al. [ 25] females with a past history of AN (followed up 10-years after in-patient treatment) seemingly have a lower percent body fat and a trend to lower serum leptin levels than BMI-matched controls.
A mutation analysis of the coding region and part of the promoter region of the leptin gene in patients with AN, followed by case control studies of the detected polymorphisms, yielded negative results [ 81].
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