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For comparison, an extra sample was prepared by directly depositing a droplet of the GNP sol onto an ITO glass substrate, and GNPs remained on the surface of the substrate after evaporating the sol under ambient conditions.
Three microliters of the sample was placed on the carbon-coated copper grid, making a thin film of sample on the grid, and an extra sample was removed using the cone of a blotting paper and kept in a grid box sequentially.
As we have mentioned, at time 0, an extra sample was taken to determine biochemical parameters.
As part of this study, an extra sample was obtained for control microscopy (Auramine-O staining) and culture on Lowenstein-Jensen solid media at the Zonal Reference Laboratory at Bugando Medical Centre.
An extra sample was treated with DMSO as a vehicle control for biotin-HPDP.
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In the BOSpos patients, one extra sample was obtained within two months before the BOS diagnosis was made.
One extra sample is to be taken one week before the start of the bathing season.
Neither was there any need for informed consent procedures as no extra sampling was performed and no personal data was stored in relation to the isolates.
No extra sampling was applied for this study, thus no formal ethical approval was required.
This means that the cost for extra sampling was saved after 49 days of generic tacrolimus.
Soil type was determined by standard lithologic and pedologic analysis of sediments; for this purpose, 2 extra samples were collected per site from a depth of 30 cm (26 ).
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