Sentence examples for an extensive purification from inspiring English sources

In our study, an extensive purification of these proteases was carried out together with the characterization of temperature and pH optimum, inhibition, substrate specificity, fibrinolytic effect and partial sequencing.

However, given the toxicity of the commonly employed copper- and iron-based catalysts, the materials generally require an extensive purification process prior to use in biomedical applications, including chromatography, precipitation, and dialysis.

We next used a more extensive purification procedure designed for labile spherical virions, which involves density gradient centrifugation (see " Materials and methods").

Although a pure sample is desired for crystallization, the protein may lose its activity following a too extensive purification, since this procedure may remove some lipids that help to maintain the integrity of membrane proteins (such as in hFLAP (5-lipoxygenase activating protein from Homo sapiens, a MAPEG member) [ 31]).

Hence, extensive purification procedures become a necessity if these oils are to be used for obtaining PUFAs [ 8].

To rule out any possible contaminants, we performed extensive purification of Dex on a continuous sucrose density gradient and confirmed the presence of ULBP-1 at density gradient corresponding to Dex floatation i.e. 1.16 to 1.19 g/ml (Fig. 4B).

The accumulation levels obtained are high enough to allow delivery of an immunologically relevant dose of IL-10 in a reasonable amount of leaf material, without extensive purification.

After extensive purification of the enzyme by a series of five different chromatographic steps, a few small low abundance RNAs copurified with the activity.

Extensive purification of the enzyme revealed a 105-kDa protein encoded by a nuclear gene termed RPM2 [66].

It is therefore possible that a higher specific activity might be reached with a non His-tagged CgChoA and after more extensive purification.

This is important because reaction products that may be entered into a chemical registration system are almost always mixtures unless extensive purification is done.