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One unit of an enzyme was defined as the amount consuming 1 μmol of the substrate per min under the below-mentioned standard assay conditions.
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The connectivity of an enzyme is defined as the number of edges connecting the enzyme to other enzymes.
When metabolite X (or metabolite Y) reacts with an enzyme as a substrate or a product, the enzyme was defined as a "reaction" enzyme.
One unit (U) of the enzyme was defined as the amount of protease that produces an increment of one absorbance unit per minute in the assay conditions (Bruno et al. 2003).
The activity of enzyme was defined as the amount of enzyme to produce 1 μmoL PED in a minute.
One unit of enzyme was defined as the amount of enzyme that liberates 1 μmole of p-nitroaniline from the substrate in 1 minute.
One unit of enzyme was defined as the amount of enzyme producing 1 μmol of NADPH per min at 25°C in the reductive reaction of methylglyoxal.
One unit of enzyme was defined as the amount catalyzing the formation of 1 μmol of NADPH/min at 50°C during meso-DAP oxidation.
One unit of the enzyme was defined as the amount required to liberate 1 μmol of p-nitrophenol under standard assay conditions.
One unit of enzyme was defined as the activity producing 1 μmol of p-nitrophenol per min under the assay conditions.
To demonstrate its use, a data set of atoms for superimposition of the HIV-1 reverse transcriptase enzyme was defined using the coordinates for the 21 available crystal structures of this enzyme and its complexes.
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