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Nevertheless, an efficient transformation of all alkyl levulinates into γ-valerolactone is possible.
X-ray diffraction and Raman spectra show an efficient transformation of the t-ZrO2 at the surface that exhibit the typical yellow-gold color of ZrN with high hardness and wear resistance.
Nair et al. (2013) described an efficient transformation of DCF by immobilized laccase of Coriolopsis gallica in a continuous stirred tank membrane reactor, though the reactor volume was only 50 ml (flow rate: 40 ml/h).
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Although still very premature, the growing interest from industry, the multiple particular phenotypes, the recent (independently performed) sequencing of three complete genomes, the development of an efficient transformation protocol, and the subsequent first transformation of B. bruxellensis (Miklenic et al., 2013) are all hinting toward an upcoming field of Brettanomyces engineering.
Lipase produced by Penicillium expansum is widely used in laundry detergent and leather industry; however, the absence of an efficient transformation technology sets a major obstacle for further enhancement of its lipase productivity through advanced gene engineering.
However, investigation of these features and validation on their biological functions are limited due to the lack of an efficient transformation method.
This is partly due to the lack of an efficient transformation system and partly due to the large genome size with an estimated 1C genome weight of 13.23 pg, corresponding to about 13,000 Mbp [ 18].
Some environments exhibit a moderately efficient transformation of TCS into MeTCS as well as other unknown transformation products, even without deliberate attempts to optimize these processes.
Together with our process for the conversion of fructose to HMF as a sole product, reaching a yield that exceeds the best performance of all systems reported in the literature and even surpassing their performance in corresponding desired solvent media, this strategy thereby affords an attractive route for highly efficient transformation of biomass to a versatile platform compound.
E. coli AU1 is a useful intermediate host for efficient transformation of C. glutamicum.
In this system, an acid was formed by the photoreaction of PAG, and then chemically amplified deprotection proceeded during postbaking, in which a large number of repeated chemical reactions were induced by a single photochemical event, resulting in the efficient transformation of protected functional groups.
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