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It's an easy tag line.
Since the pitcher is usually the best thrower on the team, stealing a base is very dangerous: the pitcher can turn and throw the ball to a baseman instead of the batter, allowing an easy tag out.
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But where Fonda's Captain America "went looking for America and couldn't find it anywhere," in the words of an "Easy Rider" tag line, Sagal finds it everywhere he goes.
A translation grade S-methionine was used (EasyTag Methionine L-[S], PerkinElmer, Waltham, MA, USA).
No easy tag exists to describe Mr. David's music.
There's no easy tag line for "the oughts," because there's no immediately recognizable constellation of values.
Fuck easy tag names and fuck commercial marketing schemes.
p53 protein translation was measured in U2OS cells that were labeled with 0.4 mCi L-[35S]methionine and L-[35S]cysteine (Easy Tag EXPRESS, Perkin Elmer) for 15 minutes, followed by immediate cell lysis in ice-cold 0.5% NP40 lysis buffer.
Cells were cultured in DMEM with 10% FBS and 10 µl/ml S-methionine/cysteine (EasyTag 35S labeling mix, PerkinElmer) for 25 min at 37°C.
For protein labeling, FT293 cells were incubated with methionine-cysteine free DMEM supplemented with 5 μl Easy Tag EXPRESS [S] Protein Labeling mix (Perkin Elmer, Waltham, MA, USA) for 30 min.
Briefly, after treatments, cells were incubated with L-[S]methionine and L-[S]cysteine (Easy Tag EXPRESS; NEN/Perkin-Elmer) for 20 min and radiolabel incorporation was monitored by resolving cell lysates on SDS-PAGE followed by transfer onto PVDF membranes and visualization with a PhosphorImager (GE Healthcare).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com