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Protein content was adjusted to 20 micrograms in all samples, and loaded in a SDS-polyacrylamide gel containing gelatin, electrophoresed and incubated overnight in an buffer (150mM NaCl, 5mM CaCl2, 50mM Tris-HCl, pH 7.6).
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"We act as a buffer, a filter, as help.
We made a buffer, a fuel-break to hold the indirect line.
The first picture shows a woman at a buffer table.
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