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The performance of the short peptide not only implies that the methodology employed here can contribute toward development of novel techniques for protein design, but it also yields insights into the raison d'etre of an autonomous element involved in a natural protein.
A full-length functional L1 element is about 6 kb in length and able to code for enzymes which are required for L1 retrotransposition, making the L1 an autonomous element [3].
The internal deletions of an autonomous element in MITE formation fall in the category of chromosome microdeletion.
It is believed that an autonomous element often gives rise to one MITE family in a genome.
An element that can produce transposases, proteins required for transposition, to mobilize itself is an autonomous element.
The Ac or Activator is an autonomous element carrying a transposase gene encoded between the cis-required terminal sequences.
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Tgmt* expressed a 14.6 kb Gene-1 that encodes the two functions required of an active, autonomous element: a transposase with a conserved Tnp2 domain and a mosaic transcript bearing little homology to other transposon mosaic gene products.
It was proposed that abortive gap repair (AGR) of the donor site of an excised autonomous element may be involved in the generation of MITEs (Feschotte et al. 2002).
Moreover, within these two categories, TEs can be also classified as active or inactive based in their actual mobility, which depends on several factors, among which the presence of an active, autonomous element of the same family--or close enough to be able to mobilize it--is fundamental.
While it is unclear whether this transposase is part of a complete autonomous element (it lies within a short 1975-bp contit), it may be a source of functional protein capable of mobilizing other OC1 copies.
A second autonomous element was also found with high similarity to an element recently described from the little brown bat, Myotis lucifugus, further implicating horizontal transfer in the evolution of this genome.
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