Sentence examples for an assembled transcriptome from inspiring English sources

Nonetheless, one of the most important uses of an assembled transcriptome is for differential expression analysis.

Through RNA-Sequencing we obtained an assembled transcriptome of 82,331 loci.

Genome-wide expression studies of marine medaka require an assembled transcriptome, and this sequencing effort has generated a valuable resource of coding DNA for a non-model species.

We have produced an assembled transcriptome for C. maenas that provides a significant molecular resource for wide ranging studies in this species.

We produced an assembled transcriptome for C. maenas that consists of 153,699 loci and 212,427 transcripts and provides a significant molecular resource for wide studies into both basic and applied biology for this species.

All sequences passing these filters were then BLAST searched against the proteins predicted in the M. incognita genome sequence [ 21] and against an assembled transcriptome derived from an RNAseq dataset of G. rostochiensis J2 and parasitic nematodes at 14 dpi (S. Eves van den Akker, unpublished).

Considering that an OHR value of 0 indicates a poor assembly and a value of 1 indicates a fully assembled transcriptome [ 33], our assembly for A. glycines seems to be fairly comprehensive.

An ortholog ratio of one indicates a fully assembled transcriptome, while a value close to zero indicates a poor assembly [ 29].

A second blast search was performed, with similar parameters, against a previously assembled transcriptome of an adult Trioza nov. sp. 1 psyllid, assembled in Trinity (Michael Brewer pers. comm). in order to identify the psyllid component present in the sequenced material.

On the assumption of conserved gene length between species, an ortholog hit ratio near zero indicates a poor assembly while values near one indicate a fully assembled transcriptome [ 30].

The number of contigs found with a best BLAST hit outside of the Brassicaceae lineage strengthen the argument that one reference transcriptome might not be sufficient to fully annotate a newly assembled transcriptome where there might occur genes that are no longer present in the reference species.