This comprised transcript profiling of an array of gene transcripts involved in regulation of mitochondrial metabolism prior and post the single bout of exercise and the measure of changes in protein levels in immunoblots or activity in enzymatic assays along with morphometric estimation of cellular variables pre- and post-endurance training (Fig. 1b).
Here we show the frequent use of trans-acting control by a single neuronal splicing factor, NOVA, to regulate an array of target transcripts.
More significantly, we show also that the wp allele carrying the Tgm-Express1 spawns an array of chimeric transcripts resulting from alternative splicing events at this locus, some of which lead to novel open reading frames.
Consistent with this interpretation of the Vangl2 Lp/Lp glomerular tuft size in relation to podocyte proliferation, we found that isolated differentiating podocytes express an array of PCP transcripts, including Vangl2.
We show that the exon/intron regions of gene fragments carried by the Tgm-Express1 of the wp allele are alternatively spliced and assembled with the constitutive exons of the F3H1 gene to generate an array of chimeric transcripts encoding a variety of open reading frames (orfs).
These three RNA polymerases produce a dazzling array of transcripts using a multitude of different promoters.
Although our investigation is necessarily limited to the analysis of moderately and highly abundant mRNAs, it offers a unique opportunity to identify a diverse array of transcripts and to examine some of the greater fluctuations in transcript abundance between and among libraries.
The combination of versatility of their RNA-binding domains with structural flexibility enables RBPs to control the metabolism of a large array of transcripts.
First, the program is complex, with several hundred proteins induced to varying levels; Second, the program is robust, a large array of transcripts and their attendant proteins, are induced more than 10-fold; Third, there is both consistency and variability in the program depending on the cell type e.g.g
E2-treated males expressed a large array of transcripts that were not associated with vitellogenesis.
