We present a novel microfabricated dielectrophoretic trap designed to pattern large arrays of single cells.
A larger or denser microwell array would allow electrophoretic analysis of a larger number of single cells in the same total assay duration.
This spacing constraint presents a trade-off between PAGE separation resolution, which benefits from long separation distances, and array density, which allows analysis of larger number of single cells.
Arrays of single-cell level microchambers have been fabricated on optical fiber bundles [2], glass slides [5], [7], [8], polydimethylsiloxane (PDMS) [3], and hydrogel [6].
First, a microfabricated array designed to systematically manipulate the magnitude of substrate deformation applied to small colony of cells [9] was used, to demonstrate deposition of single cells into spatially defined cell culture environments.
On the technical side, multiple expression levels in single cells comprising a sample can be measured by the novel methods of single cell arrays, phosphocytometry and cellomics [28], [29].
The dynamic response of a detached single CMUT cell and an array of CMUT cells is predicted and analyzed for the effect of mutual radiation.
We review mathematical approaches for deciphering the data emanating from bioreporter collections, and discuss the future of single cell arrays.
Compared to traditional microfabrication techniques, MOCCA dramatically reduces the cost and turn-around time for fabrication of single cell arrays, making it affordable for researchers.
In addition, LIPA plans to build an array of fuel cells, which generate electricity by a chemical process.
