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To avoid potential crosstalk in mammalian cell lines, a lot of efforts was expended to construct an ARE that is specific and no longer inducible by the progesterone and glucocorticoid receptor [ 17– 17].
We used the slp-ARE-Luc vector because it contains four copies of an ARE that is highly specific for AR interactions.
The slp-ARE-Luc vector contains four copies of an ARE that has been shown to be specific for AR activation while minimizing the influence of glucocorticoids (Verrijdt et al. 2002).
We showed that Nrf2 up-regulated hepcidin transcription from an ARE that bound Nrf2/MafG heterodimers.
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