For the sorption experiment, an appropriate volume of PHE and PYR dichloromethane stock solution was respectively added to each tube.
The pellet was resuspended in an appropriate volume of isolation buffer, and centrifuged at 12,000×g for 8 min.
We present a new dural incision design that provides an appropriate volume of subdural space with minimal incisions.
The chloroform fractions were pooled, chloroform was removed under vacuum and the residue was re-dissolved in an appropriate volume of chloroform.
If necessary, an appropriate volume of 0.1 M HNO3 or 0.1 M NaOH solutions was used to adjust the pH of the solution.
The fluorescein was pre-dissolved in 1 mM NaOH/H2O (1:10, v/v), and then diluted to 1 mM with an appropriate volume of HBSS.
Nanoparticle test suspensions were prepared by diluting an appropriate volume of stock suspension in complete media to achieve desired testing concentrations (from 25too 500 μg/mL).
Then an appropriate volume of sample or standard Pb2+ solution was added and the final volume was made to 5 mL with distilled water.
The subsequent dilutions were performed via diluting an appropriate volume of this solution with ethanol and the procedures described under "construction of calibration graphs" were then applied.
Added an appropriate volume of medium to the flask and aliquotted the cells into three new flasks with a starting density of 0.5 × 106 cells/mL and incubated cells at 27 °C. .
Added an appropriate volume of medium to the flask and aliquotted the cells into three new flasks with a starting density of 0.5 × 106 cells/mL and incubated cells at 27 °C.
