Per well, the transfection mixture included 50 ng Renilla luciferase as an internal control, 100 ng of the appropriate CLOCK luciferase construct, and 100 ng of the p3x-FLAG-REV-ERBα expression construct.
Establishing an appropriate molecular clock for estimating split times implies a number of decisions that can greatly affect calibration.
Control signals are automatically synchronized to the appropriate clock, that is, the "full" signal is synchronized to the write clock and the "empty" signal to the read clock.
The same machine was used for EEG data collection to ensure appropriate clock synchronization between the EEG data and responses from IVA.
Bayes Factor (BF) analysis was performed in Tracer and likelihood values based on 1,000 bootstrap replicates were evaluated to select the appropriate clock model (following criteria of Kass and Raftery [ 45] and Suchard et al. [ 46]).
Third, the use of an appropriate molecular-clock calibration greatly influences the accuracy of conclusions from coalescence analysis (Ho et al. 2011).
An algorithm for assigning appropriate clocking zones to a QCA sequential circuit is proposed.
A solution to this problem would be to start the GA with several local clocks, but determining an appropriate number of local clocks is difficult.
The results show that for satellites equipped with highly stable clocks, employing an appropriate linear model constraint for the clock estimates in orbit determination can result in an improved orbit consistency as well as accuracy.
An alternative approach would be to shift the clock to an appropriate phase.
