In addition, a subset of four Huia samples were independently extracted and amplified for three loci (Pca01, Pca05, and Pca12) at an ancient DNA facility at the University of Auckland.
The stern, ancient facility loomed behind her like an overdrawn stage set.
DNA was extracted from five King Island Emu sub-fossil bones (Museum Victoria numbers B226 450 55, 55 and 57, KI01-04 in this study respectively, B226 43 was excluded from further analysis after molecular damage proved too excessive) together with a mock extraction in a dedicated ancient DNA facility at Griffith University, Nathan, Australia.
DNA was extracted from blood, tissue, and feather samples using the DNeasy Tissue kit and manufacturer protocols (Qiagen, Inc .. Museum specimens were handled in a dedicated ancient DNA facility using a modified Qiagen DNeasy Tissue kit protocol [ 23].
Three independent extracts of aurochs DNA from sample CPC98 (labelled C1, C2 and C3), each organised into three separate ∼30 µl aliquots (labelled C11 3, C21 3 and C31 3 —giving a total of nine aliquots), were prepared for high-throughput sequencing using an Illumina Genome Analyzer, in a dedicated ancient DNA facility based in the Smurfit Institute of Genetics, Trinity College Dublin.
In accordance with criteria suggested for the verification of aDNA sequences [ 47], a number of samples were extracted and sequenced at a separate ancient DNA facility at Massey University, Auckland, New Zealand.
The Paleo-DNA Laboratory is a dedicated ancient DNA facility with stringent precautions and protocols employed for the analysis of degraded DNA.
All extraction steps and PCR set up was done in a dedicated ancient DNA facility, away from any PCR or post-PCR areas.
The time-honoured traditions of Var viticulture have been given a fresh, organic impetus at Château Léoube, a state-of-the-art facility built around an ancient castle.
The samples were extracted in a laboratory specially dedicated to aDNA work at the Archaeological Research Laboratory in Stockholm, as well as in a newly built ancient DNA facility at the National Board of Forensic Medicine in Linköping that is separated from the department's PCR areas.
