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Mitochondrial haplotypes were determined from the restriction fragment profile of an amplified segment of the cytochrome oxidase gene (COIII) after restriction with EcoRI and AccI [33].
The LRSP assay is based on coupling a fluorescent dye to an amplified segment of DNA and use of MTBC lineage specific probes-conjugated to dyes that ensures real-time identification of the genotype in 2 h for every 92 samples.
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Although a short amplified segment of intron 6 was inactive, this DNA segment appeared to become active when juxtaposed to the 5' segment of the intron 1 fragment.
This amplified segment may play a role in the higher growth rate for this parasite, because the reticulocyte-binding protein may facilitate parasite invasion.
While associations to over-expressed pathways largely coincided with signature alterations, we observed strong associations to under-expressed pathways that mostly appeared on chromosome 4. PDGFRA, KIT, and KDR genes that are located on the amplified segment 4 q12 probably play an important role in tumor biology due to their increased expression of receptors and their ligands.
Tandem duplications - Fusion points are around the start and end of an amplified genomic segment, see Figure 4D.
The observed size of chromosome V GCR was consistent with that calculated for a monocentric translocation containing the broken chromosome V joined to the amplified segment of chromosome XIV (Table 2).
The size of the amplified segment ranged from 222 to 252 bp.
The length of the amplified segment was 2218 bp.
One primer pair was located within the unamplified segment, two pairs within the amplified segment.
Here 29 known genes located within the common amplified segment at 17q23 were defined.
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