Loci with allele sizes > 166 bp did not amplify consistently in the historical samples (on average 29% amplified, range: 0-100%), and were not used in the further analysis.
Primers were developed specifying the amplified range according to the contig-protein alignment boundaries using default standard PCR settings of PerlPrimer (version 1.1.12; Marshall, 2004).
A total of 249 bands were amplified ranging from 50 to 500 bp and were 100%% polymorphic.
In comparing the 468LN vs 468GFP genomes (Table 1), the median copy number increase was 3.84N (range 2.11 – 47.50), with a median size of an amplified region being 666 kb (range 8 bp–36 Mb), while the median copy number decrease was 1.23N (range 0.07 – 1.92), with a median size of deleted region of 292 kb (range 6 bp–31 Mb).
The result is a varied set of American and English songs that range from an amplified treatment of Sacred Harp hymns to a gutsy banjo- and violin-backed arrangement of the tragic ballad Whitby Lad, and fine unaccompanied harmony singing on Love Farewell.
For determination of the reference gene, a standard curve was generated for each assay using seven serial dilutions of an amplified human brain cDNA library ranging from 20 ng to 20 fg.
Secondly, the stress response in the heat-sensitive genotype is characterised by an amplified expression of a more diverse range of functional classes including transcription, photosynthesis, hormone related and protection genes which are increased, while transport and carbohydrate metabolism are reduced in expression.
John Cloud of the Alabama state troopers," came an amplified voice.
The pickup offered an amplified sound but without much metallic vibration you see with many amplified acoustics.
It was an amplified disaster.
One calls to mind an amplified heartbeat.
