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MicroRNA targets were PCR amplified (Additional file 5: Table S2) according to Warthmann et al. [ 45] and cloned into pENTR/D-TOPO (Invitrogen).
Alpha-graphyne can be regarded as an amplified graphene by inserting additional acetylene bonds into the place of C-C bonds.
Conversely we found 17 windows with too-high coverage, 13 of which are true positive hits located in the GTP cyclohydrolase I locus (Additional file 5), an amplified region already highlighted by CGH array technology [ 11].
The pickup offered an amplified sound but without much metallic vibration you see with many amplified acoustics.
It was an amplified disaster.
One calls to mind an amplified heartbeat.
Besides the mentioned discrepancies between total RNA and amplified RNA, additional variation in results is observed when the same amplified RNA sample is analyzed on different microarray platforms [5], [6].
Totally there were 32 variant sites within the 3' end amplified region (Additional file 4).
Since all of these microRNAs amplified (see Additional file 1: Figure S1) at high PCR efficiency values, it follows that 454 sequencing generates significant sequencing mistakes.
The corresponding deduced amino acid sequence (120aa) contains some of the partial aminoacid sequences obtained by mass spectrometry, thus confirming the specificity of the amplified sequence (Additional File 2, Figure S1).
Of the 288 SNP loci for which primers were developed in this study, only six were selected that should have detected differences between rainbow and cutthroat trout, and not all of those amplified reliably (Additional file 2), so the inability to clearly differentiate the two species likely results from an ascertainment bias that is a direct result of that under-sampling.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com