Primers were designed such that PCR products would cover alternative segments of an analyzed genotype-specific splicing events.
This is true both for constitutive as well as alternative segments of 3'UTRs.
Other two alternative segments of 4 and 26 amino acids, coded by exons 13 and 15, respectively, are localized in the first intracellular loop.
If the alternative ortholog does not exist in the reference dataset, either the alternative segment will match a subregion of the original transcript form (resulting in a low ortholog hit ratio), or there may be no hit at all (resulting in an undefined ortholog hit ratio).
As for both alternative splice sites and cassette exons, the inclusion junctions corresponded to the longer isoform, Ψ-value represents the inclusion level of an alternative gene segment.
In conclusion, TMEM16A 0) may represent a useful backbone, suitable for structure activity relationship studies in which progressive additions of alternative segment regions will help in elucidating their functional roles.
With a party conference just around the corner, Mr. Johnson is making himself available as an alternative to a large segment of the party that would see Mrs. May's softening stance as a betrayal.
This large number is the result of a combinatorial explosion of possible combinations of alternative segments and edges (This is even larger in case of de novo assembly where several loci are merged into cluster of connected segments if sequence is repeated).
What makes FiberTower attractive to investors is that it offers an alternative to outdated segments of existing wireless networks.
We created 5 working groups, each designing a specific segment of an alternative process that would allow preappointment BRAF/KIT testing and delivery of results.
The study of TMEM16A isoforms, having different combinations of alternative segments, appears very important to identify the domains of the protein involved in channel function and its regulation [19].
