Recognizing that the quality of genomic DNA could potentially hinder the throughput of mutation detection, we sought an alternative method of sample quantification and normalization.
An alternative method of sample collection is the use of dried urine spots (DUS), that is, a urine sample spotted on blotting paper, which allows for stabilization by drying.
Given the need for reliable information on specific sub-groups, alternative methods of sampling allowing for the selection of probability samples were developed in the past decades.
In patients with negative sputum smear or who cannot produce sputum samples, previous studies have proved efficacy of an alternative method of obtaining respiratory samples by bronchoscopy in diagnosis of TB [ 3- 8].
Participants who are unable to provide a blood sample may provide a saliva sample as an alternative method of DNA collection (Appendix 1).
Thus, the significance of these changes needs to be verified from the same sample with an alternative method of measurement that captures the phenotypic changes accompanying driver mutations.
For comparison, we consider an alternative method of reducing coherent artifacts by illuminating the sample with the entire spectral range in a single shot, corresponding to an even lower degree of coherence.
Even so, a controlled and standardized breathomic analysis seems not easily practicable for small children, and thus, urine sampling may be an alternative method of choice if shown specific.
- Proteins may also be translated in vitro or expressed in cells, and these samples may be tested in immunoblots or immunofluorescence, as an alternative method of evaluating reactivity.
We applied an alternative method of adjustment to calculate OCM, discussed previously, using blank filter values specific to the year and type of sampler (Frank NH, unpublished data).
Leasing provides an alternative method of financing.
