The quasi-potential surface was then derived by linear interpolation among the aligned trajectories.
The quasi-potential surface can then be obtained by interpolation among the aligned trajectories, yielding the epigenetic landscape with its characteristic ridges and valleys.
In each case, we estimated the time-shift (heterochrony) between the aligned trajectories and conducted simulations to estimate the significance of the shift (Yuan et al., 2011).
(C – D ) Examples of Sla2-GFP trajectories aligned using trajectory pairs affected by different color shifts.
(A ) The shift from its correct position of a trajectory aligned to its reference in the presence of systematic color aberration.
Instead, we aligned each trajectory to the target onset (time zero) and then averaged the path offset values at each time point (5 ms intervals) across trials.
Inference or logical reasoning in the design, representation, and dissemination of architectural ideas has followed a trajectory aligned with the three forms of inference as described by Charles Sanders Peirce.
Once aligned, all trajectories were averaged to obtain the average trajectory.
To provide a framework for interpreting the tracking data, we aligned the trajectories with time-resolved average membrane shapes obtained previously by CLEM (Kukulski et al., 2012), starting from a flat membrane and ending with a vesicle.
We generated aligned average trajectories and measured the average number of molecules for the proteins tagged N-terminally (GFP-Sla2) or C-terminally (Rvs167-GFP and Abp1-GFP; Figure 2A,B).
The tart "Love & Friendship," a Jane Austen adaptation, is aligned with that trajectory, casting Beckinsale as a vain Regency widow seeking a husband to ensure her wealth.
