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The absence of an associated reduction in transepithelial peptide movement implies an additional stimulatory effect of AICAR on the basolateral peptide transport system in these cells.
However, possible upregulation of growth factors in the epithelium by HIF-1α may exert an additional stimulatory force on the stromal component.
Interestingly, co-stimulation of chondrocytes with IL-1β and PAR-2-AP showed an additional stimulatory effect, particularly on Erk1/2.
Since NK cells are known to recognise both the absence of MHC class I and an additional stimulatory signal through the NKG2D receptor, we tested whether our tumour cell lines express NKG2D ligands using an NKG2D-Fc fusion protein or GITR-L-Fc followed by a fluorescently tagged anti-human Ig Ab.
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To investigate possible additional stimulatory gating processes in kinesin, we prepared by coexpression a heterodimeric kinesin derivative in which one head has the wild-type sequence and the other has a single point mutation.
A plausible answer is that, in this crowded and more complex situation, males may have to deal with additional stimulatory and reduced repellent cues.
The effect of ERRα and PGC-1β occurred at the level of box 2 (located in the −459/−352 promoter region), although PGC-1β also displayed additional stimulatory actions on the Mfn2 promoter.
Such data suggest the existence of at least one additional stimulatory RNA species beside the L region antisense fragment and help explain why EMCVΔL is an attenuated strain: the additional stimulatory RNA means that it cannot fully evade innate immune detection while the lack of the L protein means that it cannot suppress the consequences of detection.
The observation that the presence of the wild-type head in the R210K/wild-type heterodimer complements the kinetic defect in the mutant head provides a new example of such stimulatory subunit−subunit interactions and raises the possibility that additional stimulatory gating processes may exist in the mechanism of wild-type kinesin-1.
Multiple highly homologous KIR genes map to chromosome 19q, probably arising from ancestral gene duplications, and the two main resulting gene clusters have been classified as haplotypes A and B. The A group codes mainly for KIR, which inhibit natural killer cells, whereas the B group has additional stimulatory genes.
Although it remains to be established whether this putative additional stimulatory RNA species corresponds to the RF/RI (or even acts via MDA5— Figure 5 figure supplement 1), these observations suggest the need to determine to what extent different innate immune stimuli dominate at different stages of infection.
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