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High titre virus stocks were prepared by an additional passage in CRFK cells and viral titers were determined by TCID50.
After virus replication in the bats was confirmed, an additional passage of the virus was made in Leschenault rousette bats, and bat pathogenesis was investigated.
In order to equate changes in genetic diversity to phenotypic change and directly assess how levels of co-infection alter fitness landscapes, the relative fitness of 40 biologic clones was assessed for WNV CP40 following an additional passage of 24 h on mosquito cells at either an MOI of 10 or 0.01.
An additional passage (passage 2) was performed on Vero cells for all the isolates.
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Each additional passage through a rat would take seven days from inoculation of iL3 to recovery of next generation larvae in the feces (J. Lok, personal communication).
Kaag (2008) finds that Senegalese migrants in Italy without "papers" describe themselves as being "stuck" because they do not want to expose themselves to the cost and risk of an additional irregular passage.
A paired Wolbachia-free line, designated RML12.Tet, was derived by treating cells with 1 µg/ml tetracycline for 3 passages, followed by an additional 3 passages without antibiotic before the start of the experiment.
After the initial screening, transformants containing the expected knock-in were further purified by one additional passage under selection on solid medium and screened a second time by PCR to check for segregation of the PS-layerTeth39_0206 insertion.
Biosystems Engineering 98 (115 126]126] was maintained after the additional passage of the rear tyre.
This additional passage can be avoided in principle when the partial differential equations to be solved admit a formulation in terms of boundary integral equations, leading to boundary element isogeometric analysis (IGA-BEM).
Kip woke up 10 hours later and, recalling the previous day, thought to himself, 'Yesterday was fine'; Rip slept an additional 20 years and woke with memories of what he took to be the previous day and thought to himself, 'Yesterday was fine', being unaware of the additional passage of time.
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