If the tissue was ground with a mortar and pestle and does not need additional grinding, continue with the next step.
Samples were packed into an aluminum sample holder and run as received, with no additional grinding.
For one third of culture collection samples, additional grinding was performed using plastic pestle and microfuge tube.
At first sight, this increase seems low, but in the presence of a potential damage of odontoblasts after a temperature increase of only 5.5°C, the heating of an additional 1°C after ending the grinding process is certainly noteworthy.
And to get the right texture for the burger itself, he grinds in an additional measure of fat, creating a patty much juicier than the normal French ground-beef mixture.
If tissue was ground with a FastPrep, homogenize with the cold buffer for an additional 40 s.
But that didn't stop the House of Representatives on Thursday from passing a bill that would bring resettlement of Syrian and Iraqi refugees to a grinding halt by adding additional layers of bureaucracy to an already rigorous process.
The material was shade dried and ground to a fine texture in a grinding machine.
Pure chloroplasts were observed after additional washing with grinding buffer and centrifugation.
The slabs are either pressed against a grinding wheel or crushed, sometimes with additional freezing with liquid nitrogen that makes the frozen material more brittle and easier to grind or crush.
