Exact(17)
When autophagosomes fuse with lysosomes and eGFP-LC3II is degraded by lysosomal hydrolases, the LC3-II moiety is degraded faster than the more stable eGFP moiety, resulting in a transient accumulation of eGFP, which serves as an additional confirmation of phagocytic flux.
This fact is an additional confirmation of kynurenine pathway.
The ion maps of both isotopes are very similar and provide an additional confirmation of identity.
The detection of such carbonate bands can then be taken as an additional confirmation of apatite formation.
Finally, the RNA-seq data also provided an additional confirmation of the high efficiency of the Dicer knockout.
The identification of an induced mutation in RS2 serves as an additional confirmation of the contribution of this gene to the seed raffinose and stachyose content [ 16].
Similar(43)
Pharyngeal staining was absent in all three agrin mutant strains, which is an additional confirmation for their lack of agrin expression (Fig. 8F H).
As an additional confirmation for the production of FGF-7 by the fibroblasts, we analyzed the gene expression profile of the cultured fibroblasts, using a DNA microarray as previously described by us [ 13].
Finally, an additional confirmation step of the phenotype of BY4741_ haa1Δ cells transformed with the sequenced suppressors was performed by repeating their growth curves in control conditions and in the presence of 60 mM of acetic acid in 96-well microplates (FilterMax F5, Multi-mode microplate reader, Molecular Devices), at 30 °C.
Additional confirmation of an enhanced survival phenotype in FETα-DN cells was obtained by DNA fragmentation analysis after 48 hrs GFDS. Figure 3B indicates that FETα cells had a time dependent increase in DNA fragmentation during GFDS (up to 7-fold at 48 h) as compared to FETα-DN cells.
As additional confirmation of a functional and polarised RPE, we analysed the distribution of components of the Bruch's membrane by immunofluorescence at 3 dpf.
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