Exact(8)
In order to minimize false SV calls supported by the assembly, the assembled contigs were aligned to the reference human genome using an additional alignment algorithm SSAHA2 [ 34], and the SVs again validated using R-SAP.
Several annotated sequences were then used for an additional alignment to check for conservation of H104.
We computed an additional alignment of the HIV-1 and SIVcpz viruses only (HIV-1/SIVcpz alignment).
We also added an additional alignment, which includes other atypical kinases as Figure 2 figure supplement 1.
While a second, allele-aware alignment increases accuracy at predicted heterozygous sites, these modest gains, still accompanied by a high rate of false discovery, require an additional alignment.
Upon every BLAST search, an additional 'Alignment Track' labeled "BLAST" gets added in the Browser for users to see the exact region of the genome aligned to the query, without any requirement for manual intervention.
Similar(52)
These sites do not need any additional alignment as they are of similar length.
In the case of SerRS and ThrRS, the respective protein family alignments were subsequently aligned in an additional profile alignment step.
Specifically, BWA-aligned genomes were subjected to an additional local alignment within GATK to improve the accuracy of SNP calling by locally realigning areas where there might be insertions or deletions (McKenna et al. 2010).
X-ray Diffraction: Bacteria were prepared following an already published procedure[ 6] with additional alignment.
Due to the fact that the results – and consequently our interpretations – of the docking procedure might be erroneous we performed an additional flexible alignment of compounds 1, 4, 5, and 6, and calculated a consensus pharmacophore model (Figure 3B).
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